[nazuna 04469] セミナーの御案内 (3 月 15 日 )Dr. Dierk Wanke
10.3.9 7:50 PMより転載
2010年3月15日(月) 10:00-12:00 東京理科大学 野田キャンパス 講義棟K209 (第1部
と第2部との間に小休憩を予定)
講師: Dr. Dierk Wanke (ドイツ・チュービンゲン大学 ZMBP - Pflanzenphysiologie)
http://www.zmbp.uni-tuebingen.de/plant-physiology/research-groups/harter
ke.html
第1部 "Novel approaches to unravel cellular signalling processes in plants"
The examination of signaling cascades in the inside of cells can only be
approximated by combining analyses on expression, interaction, modification
and localization in vivo and in vitro. Therefore, one of the most
challenging issues in biology and medicine is the acquisition of
quantitative data of subcellular processes at high spatio-temporal
resolution in living cells in their native tissue environment.
We established several methods to improve the current standards in
molecular biology: On the one hand, this is done by automated analysis of in
vivo imaging, the enhancement of imaging technology to reduce background and
complex correlation of fluorescence life time imaging data. On the other
hand, improvements in the analysis of large scale micro array expression
experiments and derived regulatory networks have enhanced our understanding
of cellular processes with spatio-temporal resolution.
While most of our knowledge has been tested on the AtGenExpress
resource information with Arabidopsis as a model organism, we extended our
experiences to large scale microarray expression and metabolite experiments
in barley, a moderate climate model organism for Poaceae. Simultaneous
sampling of grain tissues for analysis on expression and metabolite contents
enabled us to correlate metabolite patterns with gene expression
trajectories. Thus, we were able to identify several hundred candidate genes
that are possibly regulated by metabolite availabilities. Moreover,
exhaustive metadata analysis allowed us to compute a picture of gene
regulatory networks for the developing barley grain.
第2部 "Functional analysis of the plant transcription factors: Examples from
the BBR/BPC-family of GAGA-motif binding proteins"
The analysis of transcription factors (TFs) and their binding to
cis-regulatory DNA-motifs (CREs) is still challenging and crucial for the
understanding of the information flow within a cell. Qualitative data on
TF-CRE-interaction is lacking for more than 90 % of all eukaryote TFs.
Especially, the analysis of so far uncharacterized TFs harbors the highest
information content and might lead to entirely novel insights.
BBR/BPC proteins comprise a class of transcription factors that are
uncharacterized and confined to the plant kingdom. They have been identified
due to their specific binding to a conserved simple di-nucleotide sequence
repeat DNA-element (GA/TC)n.
The BBR/BPC family can be subdivided into distinct groups by
phylogenetic analysis on their conserved DNA-binding domain sequence. These
genes exhibit group-wise expression patterns that are indicative of a high
degree of functional redundancy between the group members.
Group II proteins form homo-/hetero-oligomeric structures in the
nucleus and the nucleolus, which are mediated by a novel type of coiled
coil-interaction domain. Target site analyses of putative binding motifs
suggest a role for BBR/BPC proteins in regulating other transcription factor
or hormone signalling related genes. Moreover, we provide indications that
BBR/BPC proteins are contained in higher order complexes with other
proteins, and that these are important for the correct localization of
BBR/BPCs to the nucleolus.
東京理科大学野田キャンパスは、東武野田線運河駅（つくばエクスプレス・流山おお
たかの森駅から7分）下車徒歩5分です。
http://www.tus.ac.jp/info/access/nodcamp.html
興味をお持ちの方の御来聴を歓迎致します。
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朽津 和幸 (Prof. Dr. Kazuyuki KUCHITSU)
